Proteogenomic Analysis Reveals Proteins Involved in the First Step of Adipogenesis in Human Adipose-Derived Stem Cells

Figure 2

Proteins identified in 24 h-differentiated and undifferentiated hASC. (a) A total of 3420 proteins were identified. The Venn diagram shows that 2322 proteins are common to 24 h-differentiated (ADI) and undifferentiated hASC (CT) conditions. Cells exposed to 24 hours of adipogenic cocktail have 53 exclusive proteins. Undifferentiated hASC have 92 exclusive proteins. The Venn diagram represents the number of proteins that satisfy a minimum of 2 replicates. (b) Gene Ontology analysis of the 53 uniquely identified proteins after adipogenic induction. Only biological processes with are shown. (c) Differently abundant proteins identified in hASC exposed to 24 hours of adipogenic differentiation. Blue dots represent the 33 proteins that satisfy our stringent criteria. Red dots represent proteins with no satisfying fold change cutoff and established FDR. Green dots represent proteins that satisfy only the fold change cutoff. Orange dots represent proteins that satisfy both criteria but received very low quantitative values. The five proteins with the highest and lowest abundance are named.

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