Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway

Figure 3

IGF-1R/AKT/mTORC1 signaling pathway-related mRNA and protein changes in LFCs with or without mechanical stress. Mechanical stress upregulated the activation of pIGF-1R (a, b), pAKT (a, c), and pS6 (a, d), as well as the mRNA levels of IGF-1 (e), col-I (f), and col-III (g) and the protein levels of IGF-1 (h), col-I (i), and col-III (j) in a time-dependent manner. Columns represent the of 3 samples, and each experiment was performed in triplicate. LFCs: ligamentum flavum cells; IGF-1: insulin-like growth factor 1; col-I: collagen I; col-III: collagen III. “” represents ; “ns” indicates .

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