Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway

Figure 5

NVP-AEW541 treatment. Mechanical stress in the nonstress group, the stress group, and the stress+100 ng/ml NVP-AEW541 group. NVP-AEW541 reduced the mRNA levels of col-I (f) and col-III (g), but not IGF-1 (e). col-I (i) and col-III (j) protein levels, but not IGF-1 (h) protein levels, were attenuated by NVP-AEW541. In addition, NVP-AEW541 reduced the activation of pIGF-1R (a, b), pAKT (a, c), and pS6 (a, d). Columns represent the of 3 samples, and each experiment was performed in triplicate. LFCs: ligamentum flavum cells; IGF-1: insulin-like growth factor 1; col-I: collagen I; col-III: collagen III. “” represents ; “ns” indicates .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)