Positively Correlated CD47 Activation and Autophagy in Umbilical Cord Blood-Derived Mesenchymal Stem Cells during Senescence

<div>Autophagy flux. (a) Rapamycin (R, 10 <i>μ</i>M), autophagy inducer, was treated in CD47 knockdown UCB-MSCs. Autophagy-related proteins were determined with immunoblotting analysis. Relative fold change of proteins, ATG5, ATG12, Beclin1, and LC3BII relative to LC3BI was quantified against protein levels of GAPDH. (b) Representative immunofluorescence images of LC3 with treatment of autophagy inducer or inhibitors showed significant different intensities on LC3 levels, contingent upon CD47 levels. Scale bar 20 <i>μ</i>m. 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Stem Cells International

fig5

Figure 5

Figure 5: Positively Correlated CD47 Activation and Autophagy in Umbilical Cord Blood-Derived Mesenchymal Stem Cells during Senescence 