Comparison of the Donor Age-Dependent and In Vitro Culture-Dependent Mesenchymal Stem Cell Aging in Rat Model

Figure 4

(a) Tubule formation by Matrigel-cultured ASCs from 6 mth rats, p. 3; 2 yrs. rats, p. 3; 6 mth rats, p. 30; 2 yrs. p. 30 and HUVEC cell culture as a control; (b) tubule-like structures emerging from spheroids cultured in collagen gel: ASC and HUVEC (control); mean numbers of the tubules produced by spheroids composed by ASCs from 6 mth and 2 yrs. donors, short-term (3 p.) and long-term (30 p.) in vitro culture. HUVEC spheroids served as controls. ; total length of tubules per spheroids composed by ASCs from 6 mth and 2 yrs. donors, short-term (3 p.) and long-term (30 p.) in vitro culture. HUVEC spheroids served as controls. ; mean tubule length produced by spheroids composed by ASCs from 6 mth and 2 yrs. donors, short-term (3 p.) and long-term (30 p.) in vitro culture. HUVEC spheroids served as controls. ; (c) HUVEC-produced tubules in Matrigel stimulated by supernatants from cultures of ASCs from 6 mth donors, p. 3; 2 yrs. donors, p. 3; 6 mth donors, p. 30; 2 yrs. donors, p. 30. Negative controls were HUVEC cultures in % FBS; positive controls were HUVEC cultures in % FBS.

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