Research Article

Comparison of the Donor Age-Dependent and In Vitro Culture-Dependent Mesenchymal Stem Cell Aging in Rat Model

Table 2

Comparison of the ASC parameters analyzed in young versus old cell donors and in cells derived from short-term versus long-term cultures of ASCs. Descriptions: -: parameter similar in both groups, ↑: parameter higher, or ↓: lower than in corresponding group. ACAN and COMP—chondrogenesis marker genes, analyzed by real-time PCR. All the differences are significant at least at (detailed data in text).

ParameterCell donor ageTime in culture
YoungOldShortLong

Surface markers CD29, CD90----
Cell granularity--
Growth rate (doubling time)----
Metabolic activity--
Clonogenic potential (CFU-F number)--
Adipogenic potential
Chondrogenic potential↓ (ACAN)↑ (ACAN)↓ (COMP)↑ (COMP)
Osteogenic potential--
Angiogenesis (tubule formation potential)
Angiogenesis (single spheroid summarized tubule length)
Angiogenesis (mean tubule length)
SA-β-Gal level↑↑