Research Article
METTL3-Mediated m6A Methylation Regulates Muscle Stem Cells and Muscle Regeneration by Notch Signaling Pathway
Figure 6
METTL3 regulates the mRNA translation efficiency of Notch signaling pathway components. (a) The Notch pathway genes with m6A modification. (b) The protein levels of Notch signaling pathway in the METTL3-depleted and control cells. (c) mRNA expression of Notch signaling pathway analyzed by q-PCR. (d) Polyribosome-bound mRNA-qPCR was used to detect translation efficiency. (e) The downstream gene expression of Notch signaling pathway was detected by qRT-PCR. ; ; . NC: negative control; sh-1: METTL3 shRNA-1; sh-2: METTL3 shRNA-2.
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