Research Article

The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons

Figure 1

NPC differentiation from hiPSC for the generation of mature MNs. (a) NPC form neural rosettes illustrated in a representative phase-contrast image. Scale bar, 200 μm. (b) Identity of NPC assessed by significant upregulation of genes involved in neurogenesis and downregulation of genes coding for pluripotency compared to hiPSC. Fold regulation of genes (≥2.0 or ≤ -2.0 in combination with ) is shown as means ( independent experiments). The horizontal red dashed line indicates a value of 0.05 whereas the vertical red dashed lines correspond to a fold regulation of -2.0 and 2.0, respectively. For better illustration, the range between -5.0 and 5.0 is additionally displayed.
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