The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons

Figure 5

Motoneuronal differentiation marker MNX1. (a) Representative immunofluorescent images of MNX1 (red) staining show nuclear expression in NPC and neurons of approach A and B. Cell nuclei were counterstained with DAPI (blue). Scale bars, 50 μm. (b) Detected via qRT-PCR, MNX1 was upregulated in both approaches in comparison to NPC ( independent experiments). See Figure 3 for all results. (c) Indifferent MNX1 protein expression was detected using Western Blot. Representative bands are shown from three independent experiments. Each bar represents the fold change .