The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons

Figure 6

Late motoneuronal differentiation marker ChAT and Islet-1. (a) Immunofluorescent images show nuclear ChAT expression (green) in NPC and neurons of approach A and B. Cell nuclei were counterstained with DAPI (blue). Scale bars, 50 μm. (b) In flow cytometry, cells of approach A and B revealed a higher ChAT-APC staining intensity compared to NPC. Representative images acquired during the flow stream highlight the difference in ChAT expression ( randomly selected images per group from three independent experiments). Scale bars, 10 μm. (c) Islet-1 (green) is selectively expressed in neurons of approach A and B, as shown in representative immunofluorescent images. Cell nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. (d) Approach A and B show increased Islet-1 gene expression, detected by qRT-PCR ( independent experiments). See Figure 3 for all results.