Research Article
The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons
Figure 7
Expression of Synapsin-1, Peripherin, and Smi-32 indicate neuronal functionality. (a) Representative immunofluorescent images show Synapsin-1 positive synaptic vesicles stained in green puncta as well as (b) Peripherin- and (c) Smi-32-positive neuronal filaments (green) in neurons of approach A and B, which were not detected in NPC. Cell nuclei were counterstained with DAPI (blue) (independent experiments). All scale bars, 50 μm.