Research Article

Oral Cancer Stem Cell-Derived Small Extracellular Vesicles Promote M2 Macrophage Polarization and Suppress CD4+ T-Cell Activity by Transferring UCA1 and Targeting LAMC2

Figure 3

UCA1 modulates the PI3K/AKT pathway in macrophages via targeting LAMC2. (a) The expression of UCA1, miR-134, LAMC2, PI3K, and AKT in macrophages after overexpression- (oe-) UCA1 treatment, measured by RT-qPCR. (b) The protein expression of LAMC2, PI3K, AKT, and p-AKT in macrophages after oe-UCA1 treatment, detected by Western blot analysis. The silencing efficiency of sh-LAMC2, verified by RT-qPCR (c) and Western blot analysis (d). (e) The expression of UCA1, miR-134, LAMC2, PI3K, and AKT in macrophages after oe-UCA1 and sh-LAMC2 treatment, determined by RT-qPCR. (f) The protein expression of LAMC2, PI3K, AKT, and p-AKT in macrophages, determined by Western blotting. , #. All cell experiments were repeated three times.
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