Exosomes Derived from Runx2-Overexpressing BMSCs Enhance Cartilage Tissue Regeneration and Prevent Osteoarthritis of the Knee in a Rabbit Model

Figure 3

Responses of rbACs stimulated by BMSCs-Exos and R-BMSCs-Exos, and the 0, 1, 5, and 10 indicates that 0, 1, 5, or 10 μg exosome concentrations/mL were used in the corresponding groups. (a) Gene expression changes of aggrecan, COL-II, and SOX9 after stimulation with different concentrations of BMSCs-Exos. (b, c) Cell migrated changes after stimulation with different concentrations of BMSCs-Exos. (d, e) Protein expression levels of aggrecan, COL-II, and SOX9 were detected by western blotting after stimulation with different concentrations of BMSCs-Exos, and the results of statistical analysis also shown. (f) Gene expression changes of aggrecan, COL-II, and SOX9 after stimulation with different concentrations of R-BMSCs-Exos. (g, h) Cell migrated changes after stimulation with different concentrations of R-BMSCs-Exos. (i, j) Protein expression levels of aggrecan, COL-II, and SOX9 were detected by western blotting after stimulation with different concentrations of R-BMSCs-Exos, and the results of statistical analysis also shown. This experiment was repeated three times. compared to 0; compared to 0; ^& compared to 1, ^&& compared to 1; ⁺ compared to 5; ⁺⁺ compared to 5.

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