Research Article
Electrophysiological Changes of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes during Acute Hypoxia and Reoxygenation
Table 1
TaqMan 20× assays (Applied Biosystems) used in preamplification and qPCR.
| Gene | Description | Function | Assay ID |
| BAX | BCL2-associated X | Proapoptotic | Hs00180269_m1 | BCL2 | BCL2, apoptosis regulator | Antiapoptotic | Hs00608023_m1 | HSPA1A | Heat shock protein family A member 1A | Cellular stress response | Hs00359163_s1 | MYH7 | Myosin heavy chain 7 | Sarcomeric protein | Hs01110632_m1 | PKM isoform 1 | Pyruvate kinase M1 | Glycolysis | Custom | PKM isoform 2 | Pyruvate kinase M2 | Glycolysis | Custom | SLC2A1 | Solute carrier family 2, member 1/GLUT-1 | Glycolysis | Hs00892681_m1 | VEGFA | Vascular endothelial growth factor A | Angiogenesis | Hs00900055_m1 | GAPDH | Glyceraldehyde-3-phosphase dehydrogenase | Housekeeping | Hs02758991_g1 | EEF1A1 | Eukaryotic translation elongation factor 1 alpha 1 | Housekeeping | Hs00265885_g1 | TBP | TATA-box-binding protein | Housekeeping | Hs00427620_m1 |
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