Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner In Vitro

Figure 8

HUCPVC indirect coculture with MDA-MB-231, SKBR3, and MCF7 breast cancer cells. (a–d) 1.5 × 10⁴ breast cancer cells were seeded in bottom wells with 2 × 10⁶ HUCPVC or media only (−) in transwells. CCK8 growth assays were performed at 48 hr for (b) MDA-MB-231, (c) SKBR3, and 72 hr for (d) MCF7. OD was measured at 450 nm, n = 3 per group. (e–g) 1 × 10⁶ (e) MDA-MB-231, (f) SKBR3, or (g) MCF7 breast cancer cells were seeded in serum-free media in Matrigel-coated transwells with bottom wells containing media only (−) or 1 × 10⁴ HUCPVC. Cancer cells invading through Matrigel were stained with cell tracker green and invasion was assessed after 72 hr. Relative fluorescence was read to measure invasiness, n = 3 per group. (h and i) After 120 hr in indirect coculture with HUCPVC: (h) CCND1 and (i) p53 expression by MCF7 breast cancer cells were measured. Data presented as fold change relative to untreated control MCF7 cells, n = 3 per group. . Error bars represent SD. Scare bar = 125 microns.

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