HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/β-Catenin Pathway

Figure 4

HMGB1 knockdown mitigates high glucose-induced erroneous differentiation of TSPCs. (a) Representative images of ARS staining of TSPCs after HMGB1 knockdown during osteogenic differentiation. (b) Quantification of ARS bound to the calcium nodules in TSPCs. (c–e) Relative mRNA levels of osteogenic genes in TSPCs after HMGB1 knockdown during osteogenic differentiation by qRT-PCR. (f–h) Relative mRNA levels of tendon-related genes in TSPCs after HMGB1 knockdown by qRT-PCR. A–G × 1; F–N × 40. KD, knockdown; vector, negative control vector. .

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