Astrobiological Field Campaign to a Volcanosedimentary Mars Analogue Methane Producing Subsurface Protected Ecosystem: Imuruk Lake (Alaska)

Table 2

Biodiversity from sample point T11-1 after microbial enrichment in several media and cloning and sequencing the 16S rDNA from total extracted DNA. T11-1 is the 30 cm deep sample from transect T11. Media: VFA minimal media enriched with volatile fatty acids. F: minimal media enriched with formaldehyde. P: minimal media enriched with peptone and yeast Extract, M: minimal media enriched with methanol, LB: organic media for heterotrophic aerobic bacteria. Fe²⁺: minimal media enriched with ferrous iron. N.D.: no data.

Culture	Blast result (NCBI database)	Gene bank ID number	Query coverage	Max. ident
T11-1 VFA	Unc. Proteobacterium	EF699933.1	100%	99%
	Shigella flexneri FBD002	EU009187.1	100%	99%
	Unc. Archaeon SPS46	AJ606292.1	10%	100%
	Unc. Propionibacterium 402C1	AM420143.1	100%	100%
T11-1 F	Psychrobacter sp. 9B	AY689064.1	100%	98%
	Antarctic sea water bac. BSW10170	DQ064630.1	100%	98%
T11-1 P	Unc. Archaeon SPS33	AJ606279.1	19%	100%
T11-1 M	Citrobacter koseri CP000822.1	CP000822.1	100%	92%
T11-1 LB	Psychrobacter sp. 9B	AY689064.1	100%	98%
	Antarctic sea water bac. BSW10170	DQ064630.1	100%	98%
T11-1 Fe^2+	N.D.