Research Article

Morphological Diversity among Accessions of Apple Tree (Malus × Domestica Borkh)

Table 2

Description of traits measured.

TraitMeasurement

OriginCountries were given codes ranging from 1 to 13. Australia had code 1, Canada 2, England 3, Israel 4, Denmark 5, France 6, South Africa 7, Russia 8, USA 9, Zimbabwe 10, Ireland 11, and Germany 12.
Fruit shapeMeasured on a score of 1.0–5.2 (Figure 1).
Fruit sizeMeasured in comparison to reference cultivars and on a score of 1–9 (Figure 1): a score of 1 showing an extremely small fruit and 9 showing an extremely large fruit.
Ground colourThe ground colour was determined on the ground colour of the skin of a fully mature fruit. It was measured on the score of 1–6: a score of 1 represented red colour, 2 orange, 3 cream-white, 4 yellow, 5 green-yellow, and 6 green.
OvercolourOvercolour of the skin of a fully mature fruit was determined on score of 1–6, where 1 represented orange, 2 pink, 3 red, 4 dark red, 5 purple, and 6 brown
Fruit attractivenessFruit attractiveness was determined subjectively as it varies between regions and experts and was scored on a scale of 1–9 with a score of 1 indicating extremely poor and reference cultivar being Egremont Russet and 9 showing extremely good and the reference cultivar being the Discovery.
Type of overcolourType of overcolour was determined on the score of 1–8. The score of 1 represented striped, 2 streaked, 3 mottled, 4 splashed, 5 slightly blushed, 6 washed out (faded), 7 complete overcolour, and 8 others.
Russet typeMeasured on a scale of 1–9: a score of 1 represented extremely fine, 2 very fine, 4 intermediate, 6 coarse, 8 scaly, and 9 cracked.
Russet amountMeasured as a percentage of fruit surface russetted. Russet amount was scored on a scale of 1–9: a score of 1 represented 0%, 2 represented 12%, 3 stood for 25%, 4 for 37%, 5 for 50%, 6 for 62%, 7 for 75%, 8 for 87%, and 9 for 100% russet.
Eating quality dessertThis was measured through a qualitative combined assessment of flavour, acidity, sweetness, aroma, and astringency at the optimum time of eating. This was scored on a scale of 1–9 where a score of 1 reflected the extremely poor eating quality and 9 showed extremely good eating quality.
Bitter pit susceptibilityThis was determined by randomly sampling 10 fruits per cultivar in the field and observing if they had bitter pit. A score of 0–9 was used, where a score of 0 depicted absence of bitter pit whereas 9 indicated extreme severity.
Bruising susceptibilityBruising susceptibility was determined by observing the condition of a fully mature fruit in a storage tray. It was measured on a scale of 1–9, where a score of 1 indicated extremely slight bruising while 9 showed extremely high susceptibility to bruising.
Firmness without skinFruit firmness without skin was recorded in kilograms on fruit that was just ripe on a part of the fruit from which the outer skin had been removed using a penetrometer that had an 8 mm probe. The pressure required to penetrate was scored on a 1–5 scale where 1 indicated extreme softness and 5 showed extreme firmness with reference cultivar being Granny Smith.
TextureThis was measured on the basis of the texture of the flesh of a ripe fruit. It was scored on a score of 1–9 with a score of 1 representing extremely coarse flesh and 9 showing extremely fine texture.
Maximum storage lifeMaximum storage life was determined through keeping 5 of each fruit type at room temperature and observing which one deteriorated first, and a score of 1–9 was assigned: a score of 1 indicating extremely poor maximum storage life while 9 indicated extremely good maximum storage life at room temperature.
Pest susceptibilityPest susceptibility was determined through the presence of key apple pests on the plants such as apple woolly aphid, codling moth, and the rosy apple aphid. The presence of the apple woolly aphid was given a score of 8, codling moth a score of 9, and rosy apple aphid a score of 10
Fungi susceptibilityFungi susceptibility was determined on the presence of certain types of fungi on a score of 1–3. A score of 1 indicated presence of Podosphera laucotricha (mildew), 2 presence of Venturia inaequalis (scab), and 3 Nectria galligena (canker), Phytophthora cactorum (collar rot and root rot)
Tree habitMeasured on the basis of the natural habit of branches of the untrained tree and a score of 1–9 with a score of 1 indicating extremely upright, 5 indicting spreading habit, 7 showing drooping habit, and 9 reflecting a weeping habit.
Tree vigourMeasured on a score of 1–9 with a score of 1 reflecting extreme weakness and 9 showing extreme vigour based on reference cultivars.
Flowering periodMeasured on a score of 1–9 with a score of 1 indicating extremely early and 9 showing extremely late.
Regularity of floweringMeasured on a score of 1–9 with a score of 1 indicating extremely irregular, 5 showing biennialism, and 9 reflecting extremely regular flowering habit.
Secondary floweringMeasured on a score of 1–9 with a score of 1 indicating extremely rare, 7 showing frequent, and 9 reflecting extremely frequent secondary flowering.
Self-compatibilityAssessed by artificially self-pollinating flowers on a tree. The outcome was grouped as self-compatible when fruits set or self-incompatible when fruits do not set.