Fluid Shear Stress and Fibroblast Growth Factor-2 Increase Endothelial Cell-Associated Vitronectin

Figure 1

Both shear stress and FGF-2 increased cell-associated vitronectin. HUVECs were exposed to steady laminar flow (20 dynes/cm²) or oscillating disturbed flow (4 ± 6 dynes/cm² shear stress, 1 Hz) in a cone and plate device, or 50 ng/ml FGF-2 for 24 hours. Samples were then analyzed for vitronectin by Western blot. (a) HUVEC phase contrast microscopy images following 24 hours of flow or FGF-2 (10x). (b) Vitronectin (VTN) Western blot (one sample out of three replicates), with GAPDH loading control and quantification of the 75/65 kDa band (normalized to GAPDH). , . One representative experiment of two.

(a)

(b)