Fluid Shear Stress and Fibroblast Growth Factor-2 Increase Endothelial Cell-Associated Vitronectin

Figure 2

FGF-2 increased cell-associated vitronectin in (a) HUVEC (human umbilical vein endothelial cells), (b) BBmVEC (bovine brain microvascular endothelial cells), (c) PAEC (porcine aortic endothelial cells), and (d) PSMC (porcine vascular smooth muscle cells). Cells seeded on native collagen-coated substrates were stimulated with 50 ng/ml FGF-2 for 24, 48, and 72 hours. Cell extracts were collected, and normalized protein samples were analyzed by Western blot. VTN intensity was quantified and normalized to GAPDH or β-actin and then normalized to untreated cell VTN levels at 24 hours. ^#, , and compared to those of the untreated cells. , one representative experiment of two.

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