Fluid Shear Stress and Fibroblast Growth Factor-2 Increase Endothelial Cell-Associated Vitronectin

Figure 3

HUVEC produced vitronectin mRNA, but the FGF-2-induced increased in cell-associated vitronectin which came from the serum. (a) mRNA was isolated via reverse transcriptase PCR in HUVEC which were treated with 50 ng/ml FGF-2 for 24 hours. (b) Cell-associated vitronectin was analyzed by Western blot in HUVEC treated with 50 ng/ml FGF-2 for 24 hours in EBM-2 0% FBS, 1 μg/ml mVTN, or 5% FBS. Vitronectin band intensity was quantified and normalized to GAPDH and then normalized to vitronectin levels in untreated cells in the serum-free medium. (c) Plasmin enzymatic activity was analyzed in HUVEC treated with 50 ng/ml FGF-2 for 24 hours in EBM-2 0% FBS, 1 μg/ml mVTN, or 5% FBS by the Chromozym assay. All samples were normalized to untreated cells in the serum-free medium. ^# and compared to those of the untreated cells in the serum-free medium. , one representative experiment of three.

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