Bone Organic-Inorganic Phase Ratio Is a Fundamental Determinant of Bone Material Quality

Figure 1

(a) Soft tissues were completely removed, and the bone was positioned in mold. (b) Mixed plaster of Paris was poured in. (c) After plaster consolidation, mold was removed. (d) The molded femur was transversely sliced in 30 mm thickness using a laser-guided mitre saw; bone specimens were cored from the slices using diamond coring bit of 8 mm diameter. (e) Information of each specimen, including animal, femur, anatomic site, and bone type, was recorded; the specimens were then labeled, wrapped with kitchen film, put in air-tight plastic container, and then stored in the freezer (-20°) before testing.

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