Inadvertent Transfer of Murine VL30 Retrotransposons to CAR-T Cells

Figure 7

Quantification of VL30 expression in human T-cells. (a) Bar graph showing VL30 expression relative to the endogenous β-actin gene. The qRT-PCR was performed in technical triplicate on RNA samples extracted from samples 4–8. Note that samples 4 and 8, in which VL30 genomes could not be detected (Figure 6), served as negative controls. (b) Gel electrophoresis analysis showing the DNA products of the abovementioned qRT analysis. Amplification products of RNA samples extracted from 293T cells transduced with the lentiviral vector pTK2229 (Figure 1) served as a positive control (P). Amplification products on of the β-actin mRNA are shown.

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