Research Article
Inadvertent Transfer of Murine VL30 Retrotransposons to CAR-T Cells
Table 4
Data of the qPCR assays described in Figure 4(a). The vector and the conditioned media employed on the target 293T cells are indicated. The presence or absence of 10 μM AZT at the time of transduction is indicated. DNA samples extracted from 293T cells transduced with the lentiviral vector pTK1261 served as negative controls. VL30 genome copy number per cell (GCN) in conditioned media-treated 293T cells and standard deviations (std) are shown. PCR amplification products of the endogenous RNaseP gene served as loading controls. values were determined by the 2-way ANOVA test. The experiment was performed in triplicate.
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