Comprehensive Comparison of AAV Purification Methods: Iodixanol Gradient Centrifugation vs. Immuno-Affinity Chromatography

Figure 1

Schematic diagram of the AAV purification comparison study. Suspension HEK293 cells in orbital shake flasks were used to produce recombinant AAVs by the triple-plasmid transfection method. Each batch was split in half for subsequent purification by either iodixanol gradient centrifugation or immune-affinity chromatography (POROS™ CaptureSelect™ AAVX column). The resulting vectors were then studied and characterized by both in vitro and in vivo assays.