Comprehensive Comparison of AAV Purification Methods: Iodixanol Gradient Centrifugation vs. Immuno-Affinity Chromatography

Figure 5

Transduction efficiency of AAV5-CB-eGFP measured by flow cytometry. In vitro transduction results of GM16095 cells ((a)  K; wells/group). In vivo transduction results of WT BALB/c mouse livers at week 2 post-IV-injection ((b) /group; dose = vg/mouse). A two-way ANOVA with Turkey’s multiple comparisons was performed. MFI = mean fluorescence intensity; %eGFP positive = percent positive cells of eGFP. , , , .

(a) In vitro

(b) In vivo