Comprehensive Comparison of AAV Purification Methods: Iodixanol Gradient Centrifugation vs. Immuno-Affinity Chromatography

Table 1

Comparative properties of iodixanol gradient centrifugation versus immuno-affinity chromatography.

	Iodixanol gradient centrifugation	Immuno-affinity chromatography
Purity	Poorer	Better
Yield monitor	No	Yes (A280 nm)
AAV serotypes	Independent	Depend on column type
Time-consuming	Yes (more steps: PEG precipitation)	Faster + automatic system
Impurities	Residual iodixanol & large proteins	Small proteins
Empty capsids	Less	More
Scalability	No	Yes
GMP readiness	Poor	Good