Research Article
Comprehensive Comparison of AAV Purification Methods: Iodixanol Gradient Centrifugation vs. Immuno-Affinity Chromatography
Table 1
Comparative properties of iodixanol gradient centrifugation versus immuno-affinity chromatography.
| | Iodixanol gradient centrifugation | Immuno-affinity chromatography |
| Purity | Poorer | Better | Yield monitor | No | Yes (A280 nm) | AAV serotypes | Independent | Depend on column type | Time-consuming | Yes (more steps: PEG precipitation) | Faster + automatic system | Impurities | Residual iodixanol & large proteins | Small proteins | Empty capsids | Less | More | Scalability | No | Yes | GMP readiness | Poor | Good |
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