Trypanosoma cruzi Necrotizing Meningoencephalitis in a Venezuelan HIV+-AIDS Patient: Pathological Diagnosis Confirmed by PCR Using Formalin-Fixed- and Paraffin-Embedded-Tissues

<div>Molecular detection of T. cruzi by PCR. (a) Amplification of the variable region of kDNA minicircles from trypanosomes (amplicon: 330 bp); line 1: 100 bp DNA ladder as molecular marker; line 2: positive control of DNA from human blood infected with T. cruzi; line 3: distilled water as negative control; and line 4: DNA from PF- and PE-sections of the frontal lobe SOL. (b) Amplification of a repeated sequence from T. cruzi satellite DNA (amplicon: 195 bp); line 1: positive control of DNA from human blood infected with T. cruzi; line 2: distilled water; line 3: DNA from PF- and PE-sections of the frontal lobe SOL; and line 4: 100 bp DNA ladder as molecular marker.</div>

Analytical Cellular Pathology

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Figure 5: Trypanosoma cruzi Necrotizing Meningoencephalitis in a Venezuelan HIV+-AIDS Patient: Pathological Diagnosis Confirmed by PCR Using Formalin-Fixed- and Paraffin-Embedded-Tissues

Figure 5 | Trypanosoma cruzi Necrotizing Meningoencephalitis in a Venezuelan HIV+-AIDS Patient: Pathological Diagnosis Confirmed by PCR Using Formalin-Fixed- and Paraffin-Embedded-Tissues 