Evaluation of the Selenotranscriptome Expression in Two Hepatocellular Carcinoma Cell Lines

Table 1

Parameters for RT-qPCR analysis.

Gene	Tm [°C]	Ta [°C]	Sequence
DIO1	59.8	61	AGCTTACTCTGGCTTTGCCGA (21)
			TATTACCCGTCTTCTCGCCCA (21)
DIO2	59.8	60	CTTACTCTGGCTTTGCCGAGA (21)
			CAGGATGTTCCGCTTGACTCT (21)
DIO3	59.8	60	GGTAGTTTCCCCCGCTTGTTT (21)
			TTTAGGTGCTGCTTTGAGGCC (21)
GPX1	59.8	60	TTATGACCGACCCCAAGCTCA (21)
			ATGTCAATGGTCTGGAAGCGG (21)
GPX2	57.3	58	GGAGAATGAACCCAAGCGAA (20)
			CAGGTTTGTCACAGCCAGTGAT (22)
GPX3	59.8	60	TCTCATCCCATGTCCACCATG (21)
			TGCATCCATTTGTGCCAGG (19)
GPX4	59.8	60	AGAGATCAAAGAGTTCGCCGC (21)
			TCTTCATCCACTTCCACAGCG (21)
GPX5	57.9	58	TCCTTCCACGACAATGGTTCA (21)
			TGTGACTGTGACCCCATTGCT (21)
GPX6	59.8	61	CAGAAACCCCACCTCACATGA (21)
			TGCCATGACCTGAATGCACT (20)
GPX7	57.9	56	TTGGTCCCATCATTCTTGTGG (21)
			GGCTGGTGATTCACTGGTCAA (21)
SELI	56.7	59	AAAGGCCAGGTTCCCAGAA (19)
			CAATCCTGCTGCAGTCCAAGT (21)
SELK	57.3	59	AATCAATCATCTGCGTGGCC (20)
			TGGTCAGCCTTCCACTTCTTG (21)
SELM	57.9	61	TCACGCAGGACATTCCATTCT (21)
			CCTGCACTAGCGCATTGATCT (21)
SELO	59.8	60	CGGTTGTGTTGCGTGTAGCTT (21)
			TGCACTCGAATGTCGTTCCTC (21)
SELS	59.8	56	CAGCTGCTCGACTGAAAATGC (21)
			GCATGCTGTCCCACATTTCAA (21)
SELT	57.9	58	TCAATCCCACACCATCGATCA (21)
			ACAACGAGCCTGCCAAGAAAG (21)
SELV	57.9	59	GTGGATTCGTCATTTCCCATG (21)
			TTTGAGTCTGACTGCCATCCC (21)
SEP15	59.8	59	ATCGGAGGCATGCAGAGAGTT (21)
			TCTGCAATCAGGATCCAGCTG (21)
SEPHS2	57.3	60	CGGCTCGCTTTTGTTCTGAA (20)
			TCGCGGCTTGTCAATGATC (19)
SELN	59.8	59	AGGCAGATGCTCATTGTTCCC (21)
			CCCCAAATCCAGATGCAGACT (21)
SEPX1	59.8	61	AGCGGCTGTTGCTCCATAACT (21)
			ATTTCAGCATCACCCACCCTC (21)
TrxR1	57.9	60	CACAATTGGAATCCACCCTGT (21)
			GGTTTGCAGTCTTGGCAACA (20)
TrxR2	57.9	62	AGGACATTTGCTGGTCGAAGC (21)
			GGAATCCCCTGGAAAAACGTT (21)
SEPP1	59.8	57	TAGGAGCTGATGCTGCCATTG (21)
			ATGTTCTCCTCTGCCCGAAGT (21)
SEPW1	59.8	60	GTTTATTGTGGCGCTTGAGGC (21)
			CCATCACTTCAAAGAACCCGG (21)