Comparison of the Manual, Semiautomatic, and Automatic Selection and Leveling of Hot Spots in Whole Slide Images for Ki-67 Quantification in Meningiomas

Figure 4

Two WSIs with an area of quantification inside a hot spot marked by two experts ((a) and (c): red and yellow rectangles) and chosen by the proposed automatic method ((b) and (d): black rectangles) with examples of 3 areas of quantification for each method and for both WSIs with large and small variation in Ki-67 index value calculated as percentage of immunopositive nuclei to the whole number of nuclei in presented area.

(a)

(b)

(c)

(d)