In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes

Figure 3

PA and fluorescence detection of CTPs in flow in vitro and in vivo. In vitro fluorescence PA signal traces of PKH26 dye stained exosomes (a) and microvesicles (b) from B16F10 melanoma cells in a 50 µm capillary tube. 2D analysis of exosome (c) and microvesicle (d) detection in (a) and (b). (e) In vivo PA detection of exosomes from B16F10 cells which were intravenously (i.v.) injected in a healthy control mouse. PAFC laser parameters: 10 kHz pulse rate, 8 ns pulse width, and 30–50 mJ/cm² energy fluence.

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