Estramustine Phosphate Inhibits TGF-β-Induced Mouse Macrophage Migration and Urokinase-Type Plasminogen Activator Production

Figure 3

EP inhibits TGF-β-induced uPA production. (a) RAW 264.7 cells were cultivated with serum-free media for 24 h in the presence of TGF-β at 5 ng/ml and indicated EP concentrations. Then conditioned media were subjected to the radial caseinolysis assay. Degradation areas represent uPA activity. Increased EP concentration strongly inhibits the TGF-β capacity to induce uPA production. (b) EP inhibits TGF-β-induced uPA promoter transactivation. RAW 264.7 cells were transfected with a p-4.8 murine uPA–Luc luciferase reporter plasmid. Then, cells were subjected to TGF-β 5 ng/ml treatment in the presence or absence of EP 10 μM. RLU: relative luciferase units. Representative results from three independent experiments are shown. Significant difference between treatments by t-test: and .

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