PAK5 Induces EMT and Promotes Cell Migration and Invasion by Activating the PI3K/AKT Pathway in Ovarian Cancer

Figure 1

PAK5 was highly expressed in ovarian cancer and predicted poor prognosis. (a) IHC staining of PAK5 in clinical ovarian cancer and paired noncancerous tissues ( for each group). Scale bar = 100 μm. (b) The IHC staining results were scored based on staining intensity (0–3 score) and percentage of positive cells (1–4 score). Immunoreactive score (IRS) was then calculated by multiplying intensity score and percentage of positive cells and represented the PAK5 staining score. (c) The 66 ovarian cancer cases were divided into metastasis and nonmetastasis cases. The IHC staining of these metastasis or nonmetastasis cases were scored, and the IRS staining score was shown. (d) Six cases that had distant metastasis were selected for qRT-PCR. The mRNA level of PAK5 in metastatic loci and in the primary tumor was compared. (e) The mRNA levels of PAK5 in a series of ovarian cancer cell lines were detected using qRT-PCR analysis. (f) Survival rate analysis of ovarian cancer patients that had either high or low level of PAK5. and .

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