PAK5 Induces EMT and Promotes Cell Migration and Invasion by Activating the PI3K/AKT Pathway in Ovarian Cancer

Figure 3

Reexpression of PAK5 in A2780 cells promoted epithelial-to-mesenchymal transition. (a) A2780 cells that had the least expression of PAK5 were transfected with an expression plasmid of PAK5 to reexpress it. An empty vector was also transfected into A2780 to serve as control. Western blot analysis of PAK5 expression was performed to confirm the working efficiency of PAK5 plasmid. (b) Cell morphology was shown in control (vector-transfected) and PAK5-overexpressed A2780 cells. (c) Immunofluorescence assay was performed to shown F-actin expression in A2780 cells. (d) Western blot analysis of epithelial marker E-cadherin and major mesenchymal markers such as vimentin, SMA, N-cadherin, and Twist in control and PAK5-overexpressed A2780 cells.

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