PAK5 Induces EMT and Promotes Cell Migration and Invasion by Activating the PI3K/AKT Pathway in Ovarian Cancer

Figure 4

Knockdown of PAK5 inhibited cell migration and invasion abilities in SKOV3 cells. (a, b) Wound healing assay was performed in shNC and shPAK5 transfected SKOV3 cells. Cells were initially transfected with respective shRNA and wounds were shown 18 h after scratch. And the relative migration (%), which was determined by the percentage of wound recovery area, was quantitatively analyzed for each group. (c, d) Cell migration assay was performed and the transmigrated cells were stained with crystal violet. Transmigrated cells were also manually counted for each group. (e, f) Cell invasion assay was performed and the invaded cells were stained with crystal violet. Invaded cells were also manually counted for each group. ; .

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