PAK5 Induces EMT and Promotes Cell Migration and Invasion by Activating the PI3K/AKT Pathway in Ovarian Cancer

Figure 5

Overexpression of PAK5 promoted cell migration and invasion in A2780 cells. (a, b) Wound healing assay was performed in vector and PAK5 plasmid- transfected A2780 cells. Wounds were shown 18 h after scratch. And the relative migration (%), determined by the percentage of wound recovery area, was quantitatively analyzed for each group. (c, d) Cell migration assay was performed and the transmigrated cells were stained with crystal violet. Transmigrated cells were manually counted for each group. (e, f) Cell invasion assay was performed and the invaded cells were stained with crystal violet. Invaded cells were also manually counted for each group. ; .

(a)

(b)

(c)

(d)

(e)

(f)