Research Article
Loss of BRCA1 Spontaneously Induces the Tumorigenesis in Lacrimal Gland
Figure 1
Abnormal eye phenotypes in Brca1co/coMMTV-cre mice. (a–d) Brca1co/coMMTV-cre mice that developed tumors at 9 (a), 16 (b), 17 (c), and 10 (d) months of age. Top: the mice exhibited exophthalmoses, protrusions around the eye, and opaque eyeballs. Bottom: dissected eyes and eye-associated tumors from the corresponding Brca1co/coMMTV-cre mice. (e) Representative MR scan image of Brca1co/coMMTV-cre mouse that developed a tumor at 10 months of age. Lacrimal tumor (indicated by arrow) side was expanded to the eye region while eye (e) in other side was intact. (f) Targeting construction of Brca1 deletion by cre recombinase. P1, P2, and P3 represent the primers for detection of targeted mutation and deletion. (g) PCR analysis against Brca1 using primers as indicated by the numbers. PCR products specific for Brca1 knockout (P1/P3) were detected in lacrimal tumors but not in normal lacrimal gland.
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