Research Article

LncRNA LOXL1-AS1 Promotes the Proliferation and Metastasis of Medulloblastoma by Activating the PI3K/AKT Pathway

Figure 2

Knockdown of LOXL1-AS1 inhibited cell viability and colony formation capacity in D283 and D341 cells. (a, b) Knockdown efficiency of two synthesized shRNAs against LOXL1-AS1 (termed as shRNA1 and shRNA2) was assessed in D283 cells and D341 cells, respectively. (c, d) After knockdown of LOXL1-AS1 in D283 and D341 cells, cell proliferative rates were monitored in a consecutive of 5 days. The absorbance on day 1 was set as 1 for each group of cells. (e, f, g) Control and LOXL1-AS1-depleted cells were subject to colony formation assay in D283 cells and D341 cells. Formed colonies were stained with crystal violet, and all colonies in each group were manually counted and averaged from three independent assays. ; .
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