In-Depth Characterization of Mass Spectrometry-Based Proteomic Profiles Revealed Novel Signature Proteins Associated with Liver Metastatic Colorectal Cancers

Figure 2

Overview of patient proteomic profiles, including normal colon (N), localized (T), and metastatic (M) CRC tissue types, as labeled at the end of each sample name. (a) Venn diagram of protein identifications from three different tissue types. (b) Principal component analysis (PCA) on the identified proteins from all samples revealed significant difference between normal colon (N) and localized (T)/metastatic (M) CRC. (c) Hierarchical cluster analysis on differentially expressed proteins among all samples. (d) Proteins with top significance () were prioritized and annotated. Most of these proteins were from extracellular regions and participate in immune activities. (e) Correlation analysis of all samples. Results showed that proteomic profiles within normal tissue (N) samples were highly correlated. However, very poor correlation was found among metastatic samples which indicated the high heterogeneity of metastatic CRC.

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