MicroRNA-19a Targets Fibroblast Growth Factor-Inducible Molecule 14 and Prevents Tubular Damage in Septic AKI

Figure 2

miR-19a inhibits the LPS-induced tubular apoptosis. (a) Murine MCT and RAW 264.7 cells with negative control (NC) transfection, miR-19a mimic (miR-19a) transfection, or miR-19a mimic (miR-19a) transfection plus zVAD-FMK treatment were separately stimulated with the indicated concentrations of LPS (L) for 72 h, and the cell viabilities were measured by MTT assay. versus L+NC, one-way ANOVA, post hoc comparisons, and Tukey’s test. D: DMSO. (b) DEVDase activity of murine MCT and RAW 264.7 cells with negative control (NC) transfection, miR-19a mimic (miR-19a) transfection, or miR-19a mimic (miR-19a) transfection plus zVAD-FMK treatment in the presence or absence of 150 ng/mL LPS (L) stimuli for 72 h. versus L+NC, one-way ANOVA, post hoc comparisons, and Tukey’s test. (c) Representative pictures and quantification from TUNEL- and cleaved caspase-3- (c-caspase-3) staining assay of murine MCT and RAW 264.7 cells with negative control (NC) transfection, miR-19a mimic (miR-19a) transfection, or miR-19a mimic (miR-19a) transfection plus zVAD-FMK treatment in the presence or absence of 150 ng/mL LPS (L) stimuli for 72 h. versus L+NC, one-way ANOVA, post hoc comparisons, and Tukey’s test.

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