MicroRNA-342 Promotes the Malignant-Like Phenotype of Endometrial Stromal Cells via Regulation of Annexin A2

Figure 3

miR-342 targets and inhibits ANXA2 expression. (a) The binding sites between miR-342 and ANXA2 predicted by a bioinformatics website (http://starbase.sysu.edu.cn/). (b) The targeting relationship between miR-342 and ANXA2 validated using dual-luciferase reporter assays. (c) The mRNA expression of ANXA2 determined using RT-qPCR after miR-342 was overexpressed or inhibited in cells. (d, e) The protein expression of ANXA2 determined using Western blot analysis after miR-342 was overexpressed or inhibited in cells. (f) The mRNA expression of ANXA2 in normal () and ectopic endometrial tissues () assayed using RT-qPCR. (g) The expression correlation between miR-342 and ANXA2 analyzed by Pearson’s correlation analysis. vs. NC mimic; ^#vs. NC inhibitor. ^&vs. normal endometrial tissues. All data, representative of three independent experiments in triplicate, are shown as . Unpaired -test was adopted to analyze the differences between two experimental groups. One-way ANOVA, followed by Tukey’s multiple comparison test, was performed to test data among multiple groups. RT-qPCR: reverse transcription quantitative polymerase chain reaction; miR: microRNA; ANXA2: Annexin A2; NC: negative control.

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