Research Article
Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis
Figure 3
STAT3 activates transcription activity of miR-19a in the hypoxia-exposed CRC cells: (a) possible upstream regulators of miR-19a predicted using TransmiR v2.0; (b) five segments of miR-19a promoter divided from the distal side to near side; (c) luciferase activity of the miR-19a promoter segments in HEK293T cells after hypoxia exposure (, two-way ANOVA); (d) binding relationship between STAT3 and the miR-19a promoter validated using a ChIP-qPCR assay (, two-way ANOVA); (e) mRNA expression of STAT3 in tumor tissues and the paracancerous tissues examined by RT-qPCR; (f) a positive correlation between STAT3 and miR-19a in CRC tumor tissues (, Pearson’s correlation analysis); (g) phosphorylation of STAT3 in HCT116 and SW480 cells after hypoxia exposure examined by western blot analysis. Data were presented as the from three independent experiments.
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