Research Article

Hypoxia Enhances Activity and Malignant Behaviors of Colorectal Cancer Cells through the STAT3/MicroRNA-19a/PTEN/PI3K/AKT Axis

Figure 3

STAT3 activates transcription activity of miR-19a in the hypoxia-exposed CRC cells: (a) possible upstream regulators of miR-19a predicted using TransmiR v2.0; (b) five segments of miR-19a promoter divided from the distal side to near side; (c) luciferase activity of the miR-19a promoter segments in HEK293T cells after hypoxia exposure (, two-way ANOVA); (d) binding relationship between STAT3 and the miR-19a promoter validated using a ChIP-qPCR assay (, two-way ANOVA); (e) mRNA expression of STAT3 in tumor tissues and the paracancerous tissues examined by RT-qPCR; (f) a positive correlation between STAT3 and miR-19a in CRC tumor tissues (, Pearson’s correlation analysis); (g) phosphorylation of STAT3 in HCT116 and SW480 cells after hypoxia exposure examined by western blot analysis. Data were presented as the from three independent experiments.
(a)
(b)
(c)
(d)
(e)
(f)
(g)