Pannexin1: Role as a Sensor to Injury Is Attenuated in Pretype 2 Corneal Diabetic Epithelium

Figure 3

Pannexin1 mediates communication between corneal epithelial cells. (a) Representative images of B6 control corneas incubated in the presence of 10Panx or a scrambled control (control) peptide and then labelled with Cal-520 AM and CellMask Deep Red for imaging. Representative images of the Ca²⁺ wave in basal epithelial cells after injury: 50, 100, and 150 seconds. Arrowheads mark cells to follow over time. (b) MATLAB event charts showing the Ca²⁺ mobilization events detected over time. (c) Event probability values comparing corneas preincubated in the presence of either 10Panx or scrambled control (control) peptide followed by an epithelial scratch injury. (d) Event probability values comparing the wound response near the leading edge of the corneal epithelium ex vivo to in vitro following a scratch wound. Data are expressed as and were analyzed with a one-way ANOVA with the Tukey’s multiple comparison test. . is a minimum of 3 independent experiments.

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