MicroRNA-137-3p Improves Nonalcoholic Fatty Liver Disease through Activating AMPKα

Figure 4

miR-137-3p antagomir promotes hepatic oxidative stress and inflammation in HFD mice. (a) Mice were fed with a HFD for 24 weeks to establish NAFLD and were also intraperitoneally injected with the miR-137-3p antagomir or antagomir control (100 mg/kg weekly) at the last 6 consecutive weeks. Relative hepatic ROS level determined by DCFH-DA probe (). (b) Relative H₂O₂ level in the liver (). (c) MDA generation in the liver (). (d) GSH level in the liver (). (e) Total SOD and CAT activity in the liver (). (f–h) Hepatic IL-1β, IL-6, MCP-1, TNF-α, and IL-10 levels determined by the commercial ELISA kits (). Data were expressed as the , and was considered significant.