LINC00665 Facilitates the Malignant Processes of Osteosarcoma by Increasing the RAP1B Expression via Sponging miR-708 and miR-142-5p

Figure 3

LINC00665 operates as a miRNA sponge for miR-708 and miR-142-5p. (a, b) FISH assays and qRT-PCR assays determined the subcellular localization of LINC00665, miR-708, and miR-142-5p in OS cells. (c) Predicted binding sequences of miR-708 or miR-142-5p with LINC00665. (d, e) Expressions of miR-708 and miR-142-5p in OS tissues and cell lines detected by qRT-PCR. (f) RIP assays identified the enrichment of LINC00665, miR-708, and miR-142-5p in Ago2 conjugates. (g) Predicted binding sequences of miR-708 or miR-142-5p with LINC00665 were validated by luciferase reporter assays. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)