LINC00665 Facilitates the Malignant Processes of Osteosarcoma by Increasing the RAP1B Expression via Sponging miR-708 and miR-142-5p

Figure 4

LINC00665 upregulates the RAP1B expression via miR-708 and miR-142-5p. (a) Predicted binding sequences of miR-708 or miR-142-5p with 3UTR of RAP1B mRNA. (b) Predicted binding sequences of miR-708 or miR-142-5p with 3UTR of RAP1B mRNA were validated by luciferase reporter assays. (c) The efficiency of the overexpression and knockdown of miR-708 and miR-142-5p in OS cells detected by qRT-PCR. (d) Western blot assays indicated the impact of miR-708 and miR-142-5p on the protein level of RAP1B, as well as the impact of LINC00665 together with miR-708 or miR-142-5p on the protein level of RAP1B. (e) Expressions of RAP1B mRNA in paired OS and nontumorous normal tissues were detected by qRT-PCR. (f) Expressions of RAP1B mRNA in OS cell lines and normal osteoblast cell line were detected by qRT-PCR.

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