Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer

Figure 2

Knockdown of KDM1A contributes to the suppressed cell proliferation and migration of cervical cancer cells. (a) mRNA expression level of KDM1A in HeLa cells after KDM1A knockdown was determined by qRT-PCR; (b) protein expression level of KDM1A in HeLa cells after KDM1A knockdown was determined by western blot; (c) mRNA expression level of KDM1A in SiHa cells after KDM1A knockdown was determined by qRT-PCR; (d) protein expression level of KDM1A in SiHa cells after KDM1A knockdown was determined by western blot; (e) CCK8 assay was applied to assess the effect of KDM1A knockdown on proliferation of HeLa cells; (f) CCK8 assay was applied to assess the effect of KDM1A knockdown on proliferation of SiHa cells; (h, h) cell clone formation assay was used to assess the effect of KDM1A knockdown on cell clones in HeLa cells and SiHa cells; (i, j) wound healing assay analyzed the effect of KDM1A knockdown on cell migration in HeLa and SiHa cells. and , compared with the control group.

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