Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer

Figure 3

Overexpression of DACT1 leads to suppressed proliferation and migration in both HeLa and SiHa cells. (a) mRNA expression level of DACT1 in HeLa cells after DACT1 overexpression was determined by qRT-PCR; (b) protein expression level of DACT1 in HeLa cells after DACT1 overexpression was determined by western blot; (c) mRNA expression level of DACT1 in SiHa cells after DACT1 overexpression was determined by qRT-PCR; (d) protein expression level of DACT1 in SiHa cells after DACT1 overexpression was determined by western blot; (e) CCK8 assay was applied to assess the effect of DACT1 overexpression on proliferation of HeLa cells; (f) CCK8 assay was applied to assess the effect of DACT1 overexpression on proliferation of SiHa cells; (g, h) cell clone formation assay was used to assess the effect of DACT1 overexpression on cell clones in HeLa cells and SiHa cells; (i, j) wound healing assay analyzed the effect of DACT1 overexpression on cell migration in HeLa and SiHa cells. , , and , compared with the control group.

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