Histone Deacetylation Regulated by KDM1A to Suppress DACT1 in Proliferation and Migration of Cervical Cancer

Figure 5

Overexpression of DACT1 reverses the promotive effect of KDM1A overexpression on cervical cancer cells. (a) mRNA expression levels of KDM1A and DACT1in HeLa cells by qRT-PCR; (b) protein expression levels of KDM1A and DACT1in HeLa cells by western blot; (c) mRNA expression levels of KDM1A and DACT1in SiHa cells by qRT-PCR; (d) protein expression levels of KDM1A and DACT1in SiHa cells by western blot; (e) CCK8 assay to measure the cell proliferation ability in HeLa cells; (f) CCK8 assay to measure the cell proliferation ability in SiHa cells; (g) cell clone formation for cell clone count in both HeLa and SiHa cells; (h, i) wound healing assay to measure the migration ability of HeLa cells; (j, k) wound healing assay to measure the migration ability of SiHa cells; and . ns: not significant.

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