circ-SIRT1 Promotes Colorectal Cancer Proliferation and EMT by Recruiting and Binding to eIF4A3

Figure 5

circ-SIRT1 promotes EMT by binding to eIF4A3. RIP and RNA pull-down assays demonstrated the binding of circ-SIRT1 to eIF4A3 (a) and (b). The expression of EIF4A3 was not regulated by cirC-SIRT1 (c). The expression of circ-SIRT1 after transfecting HCT116 cells with overexpression vector (d). Anti-IgG antibody was used as a negative control. After overexpression of circ-SIRT1, enrichment of EMT-related proteins mRNA in immunoprecipitation of anti-EIF4A3 RNA (e) and (f). Downregulation of EIF4A3 expression promotes the expression of EMT-related proteins N-cadherin and vimentin (g) and (h). Knockdown of EIF4A3 can reverse the inhibitory effect of circ-SIRT1 on EMT (i). Schematic diagram of circ-SIRT1 combined with eIF4A3 to regulate the proliferation of colorectal cancer cells (j). stands for , stands for .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)